N5-Methyltetrahydrofolate-Homocysteine Transmethylase

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N5-methyltetrahydrofolate-homocysteine transmethylase. Propylation characteristics with the use of a chemical reducing system and purified enzyme.

A simple method is described whereby millimicromole amounts of extensively purified N5-methyltetrahydrofolatehomocysteine transmethylase (vitamin Blz transmethylase) can be chemically propylated to yield an inhibited vitamin Blz enzyme which is reactivated with light. The procedure consists of a short incubation of the enzyme with propyl iodide in the presence of reduced flavin mononucleotide a...

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Mechanism of decrease in serum folate levels of rats receiving diphenylhydantoin.

Decreased serum folate levels of patients receiving anticonvulsants have been well documented (Klipstein 1964; Reynolds 1968; Dahlke and Mertens-Roeslei 1967; Horwitz et al. 1968; Jensen and Olesen 1969; Houben et al. 1971). At present time, however, the mechanism for this abnormality seems to be remaining unsolved, even though several attractive theories have been advanced, that is, an increas...

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Characterization of N5-methyltetrahydrofolate uptake in cultured human retinal pigment epithelial cells.

PURPOSE To determine the identity of the transport process that is responsible for the uptake of N5-methyltetrahydrofolate, the predominant form of folate in blood, into cultured human retinal pigment epithelial cells. METHODS Human retinal pigment epithelial cells were cultured on an impermeable plastic support, and the characteristics of the uptake of radiolabeled N5-methyltetrahydrofolate ...

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Thetin-homocysteine transmethylase; some further characteristics of the enzyme from rat liver.

Cuttle, T. D. (1938). Quart. J. Med. 31, 575. Franke, M. & Horwitz, S. (1938). Z. G(eburt8h. Gynuk. 103, 318. Hagtvet, J. (1945). Skr. nor8ke Viden8kAkad. 2, 1. Quoted in Chem. Abstr. (1947). 41, 796c. Hess, A. F. (1920). Scurvy-Pa8t and Pre8ent. Philadelphia: Lippincott and Co. Humphrey, J. H. & Jaques, R. (1954). J. Phy8iol. i24,305. Lawrynowicz, A. (1931). J. Phy8iol. Path. gin. 29, 270. Min...

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Role of polymorphism of methyltetrahydrofolate-homocysteine methyltransferase (MTR) A2756G and breast cancer risk.

Breast cancer (BC) is one of the most common causes of death among women, and second in Iran. The objectives of this study were to determine the frequency of methyltetrahydrofolate-homocysteine methyltransferase (MTR) 2756 gene polymorphism in patients with breast cancer. For the first time, we evaluated these polymorphisms and effects on the breast cancer risk association in an Iranian sporadi...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1967

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)96120-x